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KMID : 0606320040210010073
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Journal of Phamacetical Sciences Sookmyung Women s University
2004 Volume.21 No. 1 p.73 ~ p.78
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Effects of pear (Pyrus pyrifolia) methanol extracts on the production of nitric oxide and tumor necrosis factor-α in mouse peritoneal macrophages
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Hwang Yoo-Kyung
Pyo Myoung-Yun
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Abstract
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This study was conducted to investigate the effects of pear methanol extracts (P-M) on mouse peritoneal macrophages. Peritoneal macrophages were isolated from mice and cultured with or without LPS in the presence of P-M(0.16,0.31,0.63,1.25,2.50mg/m1) for 48hrs (TNF-α) or 96hrs (NO). NO levels in culture supernatant were determined by measuring the amount of nitrite, the stable oxidation product of NO, and TNF-α levels in culture supernatant were assayed by a sandwich ELISA. P-M significantly stimulated NO and TNF-α production from peritoneal macrophages at all doses. However, LPS-inducted NO production was inhibited by P-M in a dose dependent manner and LPS-induced TNF-α production was not altered by P-M.
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